A comprehensive evaluation of histopathology foundation models for ovarian cancer subtype classification.

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All Authors

Breen, J.
Allen, K.
Zucker, K.
Godson, L.
Orsi, NM.
Ravikumar, N.

LTHT Author

Allen, Katie
Zucker, Kieran
Godson, Lucy
Orsi, Nicolas

LTHT Department

Pathology
Histopathology
Oncology
Leeds Cancer Centre
Research & Innovation

Non Medic

Digital Pathology Scientist

Publication Date

2025

Item Type

Journal Article

Language

Subject

Subject Headings

Abstract

Histopathology foundation models show great promise across many tasks, but analyses have been limited by arbitrary hyperparameters. We report the most rigorous single-task validation study to date, specifically in the context of ovarian carcinoma morphological subtyping. Attention-based multiple instance learning classifiers were compared using three ImageNet-pretrained encoders and fourteen foundation models, each trained with 1864 whole slide images and validated through hold-out testing and two external validations (the Transcanadian Study and OCEAN Challenge). The best-performing classifier used the H-optimus-0 foundation model, with balanced accuracies of 89%, 97%, and 74%, though UNI achieved similar results at a quarter of the computational cost. Hyperparameter tuning the classifiers improved performance by a median 1.9% balanced accuracy, with many improvements being statistically significant. Foundation models improve classification performance and may allow for clinical utility, with models providing a second opinion in challenging cases and potentially improving the accuracy and efficiency of diagnoses.

Journal

Npj Precision Oncology