Insertional diversification of the immunoglobulin heavy chain in chronic lymphoproliferative disorders.

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All Authors

Fisher, A.
Shingles, J.
Newton, D.
Tooze, R.

LTHT Author

Tooze, Reuben

LTHT Department

Oncology
Leeds Cancer Centre
Haematological Malignancy Diagnostic Service

Non Medic

Publication Date

2025

Item Type

Letter

Language

Subject

Subject Headings

Abstract

Insertional diversification of the immunoglobulin heavy chain (IgH) switch and variable regions occurs when nucleic acid sequences from non-IgH sites are integrated into these regions. This unconventional antibody diversification mechanism could contribute to lymphomagenesis. We present an analysis of IgH/BCR VDJ (IGHV) sequencing data to reveal insertion events in chronic lymphoproliferative disorder patients and the development of a targeted switch region sequencing approach. The targeted switch region sequencing successfully demonstrated inserts in polyclonal cells, and we describe insertional diversification events occurring in the variable region in chronic lymphoproliferative disorders.

Journal

British Journal of Haematology

URI

https://hdl.handle.net/20.500.14838/2084

DOI

https://dx.doi.org/10.1111/bjh.20139

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